在线投稿

·

投稿咨询:024-31416894

高级检索
检索说明
NRR企业微博
数据库收录证明
本刊声明
投稿要求 Guide for Authors
更多信息 More information
· 招聘审稿人
· 年度稿约
· 手机杂志
· 网页杂志
· 修稿要求
· 样例稿件
· 作者承诺书
· 以往目次
· 2017年目次
· 2016年目次
· 封面目次
· 微信分类
· 查重流程
· 英文稿约
· 杂志介绍
· 官方微信
· INRS历届会议日程
细胞图库 Cell image gallery
神经损伤与再生
神经损伤与再生
脑损伤与再生
脑损伤与再生
脊髓损伤与再生
脊髓损伤与再生
视神经损伤与再生
视神经损伤与再生
周围神经损伤与<BR>再生
周围神经损伤与
再生
神经退行性病与<BR>再生
神经退行性病与
再生
颅神经损伤与再生
颅神经损伤与再生
干细胞与神经再生
干细胞与神经再生
友情链接 Links  
· Neural Regeneration Research
· 《中国组织工程研究》杂志
· 国际会议(中文)
· 国际会议(英文)
· 国际服务
· 辽宁细胞生物学学会
· 骨科临床试验
· 退行性病临床试验
· 医学气体
· 国内学会
主  管:中华人民共和国国家
     卫生和计划生育委员会
主  办:中国康复医学会
     ISSN 1673-5374
     CN 11-5422/R
投稿咨询:bwb01@nrren.org
     024-31416894
审稿咨询:024-31416894
值班电话:18704095275
社长信箱:szb@nrren.org
     024-31416691
神经损伤与再生  

脑缺血再灌注降低大脑皮质中前蛋白转化酶2的活性一文的图片
(Volume 8 Number 1 January 2013)


Cite this article:
Shuqin Zhan, An Zhou, Chelsea Piper, Tao Yang. Dynamic changes in proprotein convertase 2 activity in cortical neurons after ischemia/reperfusion and oxygen-glucose deprivation[J]. Neural Regeneration Research, 2013, 8(1): 83-89.

 


Quantitative analysis of experimental animals

This experiment is composed of an in vivo study and an in vitro study. For the in vivo study, a total of 18 Sprague-Dawley rats were initially included in the experiment, and were equally and randomly divided into three groups: control, and 8 and 24 hours following reperfusion. Rats in the 8 and 24 hours ischemia/ reperfusion groups were subjected to transient focal cerebral ischemia by performing middle cerebral artery occlusion with a 3-0 silk suture. After 100 minutes of middle cerebral artery occlusion, the suture was withdrawn to allow reperfusion for a period of 8 or     24 hours. All rats were included in the final analysis. For the in vitro study, about 30 rat pups that were born within 3 days were used for cell cultures.

 

Decreased proprotein convertase 2 activity in the cortex of rats with cerebral ischemia/reperfusion injury

In the ischemic cortex after 8 or 24 hours of reperfusion, proprotein convertase 2 activity was significantly lower than in the control cortex (P < 0.05). Proprotein convertase 2 activity decreased gradually with increasing reperfusion time in the ischemic cortex (P < 0.05; Figure 1).

Figure 1 Proprotein convertase 2 activity in rat cortex following cerebral ischemia/reperfusion.

Proprotein convertase 2 activity was significantly decreased after 8 and 24 hours of reperfusion compared with the controls. Data are expressed as mean ± SEM   (n = 6). aP < 0.05, vs. control group; bP < 0.05, vs. 8 hours, by least significant difference t-test.

I: Control; II, III: 8, 24 hours after reperfusion.

Increased number of apoptotic neurons in the rat cortex following oxygen-glucose deprivation

In rat cortical neuron cultures, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling (TUNEL)-positive neurons increased significantly after 60, 120 and     180 minutes of oxygen-glucose deprivation, relative to the control (P < 0.05; Figure 2).

Figure 2  Apoptotic neurons in rat cortex following oxygen-glucose deprivation (OGD).

(A) Rat cortical neurons after 120 minutes of OGD (TUNEL staining, × 100). The cells showing green fluorescence are TUNEL-positive neurons.

(B) Percentages of TUNEL-positive neurons. Data are expressed as mean ± SEM. aP < 0.05, vs. control group, by paired t-test.TUNEL: Terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate- biotin nick end labeling.

Decreased proprotein convertase 2 activity in cultured rat cortical neurons following oxygen- glucose deprivation

Proprotein convertase 2 activity was significantly lower in cultured rat cortical neurons after 30, 60, 120 and    180 minutes of oxygen-glucose deprivation, compared with the control (P < 0.05). Proprotein convertase 2 activity decreased gradually with increasing duration of oxygen-glucose deprivation in cultured rat cortical neurons (P < 0.05; Figure 3).

Figure 3  Proprotein convertase 2 activity in cultured rat cortical neurons following oxygen-glucose deprivation (OGD).

Proprotein convertase 2 activity was significantly decreased after 30, 60, 120 and 180 minutes of OGD, compared with the control group. Data are expressed as mean ± SEM. aP < 0.05, vs. control group; bP < 0.05, vs. previous time point, by least significant difference t-test.
   版权所有:《中国神经再生研究(英文版)》杂志社 京ICP备14021822号-1
《中国神经再生研究(英文版)》杂志社地址:沈阳10001邮政信箱,邮编:110180